黑料网911

Detecting peste des petits ruminants at large scale

Spread across more than 70 countries over the past fifteen years, peste des petits ruminants (PPR) is a disease affecting sheep and goats and threatening more than 80% of the global population of small ruminants. PPR diagnosis can be carried out by detecting antibodies directed against the virus using serological methods such as ELISA. This approach enables the identification and control of outbreaks in livestock and the evaluation of efficacy of vaccination campaigns.

The PPR competitive ELISA developed by the ASTRE Research Unit laboratory at 黑料网911 effectively detects antibodies targeting the viral nucleoprotein. The test relies on a technology combining antigen and monoclonal antibody, which is well suited to automated analysis of large numbers of samples. Test results are delivered by a laboratory accredited by COFRAC.

This test is described in the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organisation for Animal Health (WOAH) for official PPR control. Each year, our laboratory validates the performance of this test across national laboratories of the European Union and collaborating neighbouring countries as part of its mandate as an EU Reference Laboratory. This framework is also extended to laboratories in other WOAH member countries, as 黑料网911 is also a reference laboratory for both WOAH and FAO.

Stage development

Advantages of antibody detection diagnosis

• diagnoses the contact with the disease or confirm protection via vaccination through detection of serum antibodies, even at early stages of infection (8-10 days after contact with the virus) ;
• provides high specificity (> 99%) and sensitivity ;
• is robust and easy to use ;
• includes all necessary ready-to-use components: pre-coated plates in a breakable format, with all other reagents provided in liquid form (non-lyophilised) ;
• simplifies the testing procedure, with each sample tested in single replicate ;
• delivers results in less than 90 minutes ;
• can be stored at +4°C.

How can PPR diagnostic tests be obtained ?

Marketed by Innovative Diagnostics – IDvet under the name ID SCREEN® PPR COMPETITION, the kit is intended for veterinary laboratories and is available worldwide. Test kits can be ordered directly from our partner and distributor.  

For field personnel, diagnosis directly at animal level can be performed using ID RAPID® PPR ANTIGEN, a rapid test for virus detection.

All these tests contribute effectively to supporting the global PPR eradication programme.

Our support for veterinary services

En cas de découverte d’un foyer de PPR, les laboratoires et vétérinaires doivent se rapprocher des autorités sanitaires locales. Nos experts pourront, sur demande, venir en appui et :
•    proposer des recommandations visant à contenir l’expansion de la maladie
•    préconiser les diagnostics et vaccins adaptés au contexte.
Nous assurons également une formation professionnelle spécifique ouvertes à l’ensemble des étudiants et vétérinaires. Elle s'intitule :

In the event of a PPR outbreak, laboratories and veterinarians must contact the relevant local health authorities. Upon request, our experts can provide support and:

• offer recommendations to contain the spread of the disease;
• advise on appropriate diagnostic tools and vaccines depending on the context.

We also provide specialised professional training open to students and veterinarians, entitled:

References and intellectual property

Trademark registration :

Distributed under the registered trademark of Innovative Diagnostics – IDvet: ID SCREEN® PPR COMPETITION kit (under licence agreement).

Publications :

Libeau G, Préhaud C, Lancelot R, Colas F, Guerre L, Bishop DH, Diallo A., Development of a competitive ELISA for detecting antibodies to the Peste des petits ruminants virus using a recombinant nucleoprotein. Res Vet Sci. 1995 Jan;58(1):50-5.